| 产品介绍： |
| 中文名称: |
雨蛙素 |
| 英文名称: |
Caerulein |
| 别名: |
硫酸化蓝肽 Glp-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2 |
| CAS No: |
17650-98-5 |
分子式: |
C58H73N13O21S2 |
分子量: |
1352.40 |
| MDL: |
MFCD00076478 |
产品描述：
基本信息
| 产品编号： |
C10047 |
| 产品名称： |
Caerulein |
| CAS: |
17650-98-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
| |
|
| 分子式： |
C58H73N13O21S2 |
溶于液体 |
-80℃ |
两年 |
| 分子量 |
1352.40 |
-20℃ |
一个月 |
| 化学名： |
Glp-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2 |
| Solubility (25°C)： |
体外:
|
DMSO |
100mg/mL (73.94mM) |
| Ethanol |
Insoluble |
| Water |
Insoluble |
| 体内(现配现用): |
1.请依序添加每种溶剂：Saline
Solubility: 12.5mg/mL (9.24mM); Clear solution; Need ultrasonic
2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline
Solubility: ≥ 2.08mg/mL (1.54mM); Clear solution |
| 此⽅案可获得 ≥ 2.08mg/mL (1.54mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
3.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08mg/mL (1.54mM); Clear solution |
| 此⽅案可获得 ≥ 2.08mg/mL (1.54mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 |
4.请依序添加每种溶剂：10% DMSO→90% corn oil
Solubility: 2.08mg/mL (1.54mM); Clear solution; Need warming |
| 此⽅案可获得 2.08mg/mL (1.54mM) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 |
| ＜1mg/ml表示微溶或不溶。 |
| 本司提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
| 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积
质量 |
1mg |
5mg |
10mg |
| 1mM |
0.7394mL |
3.6971mL |
7.3943mL |
| 5mM |
0.1479mL |
0.7394mL |
1.4789mL |
| 10mM |
0.0739mL |
0.3697mL |
0.7394mL |
| 50mM |
0.0148mL |
0.0739mL |
0.1479mL |
生物活性
| 产品描述 |
一种胆囊收缩素（CCK）的肽类类似物，运用于大鼠、小鼠、狗和叙利亚仓鼠等动物急性胰腺炎（AP）模型的建立。建模机制在Caerulein 雨蛙肽（雨蛙素）能刺激胰腺，促使胰蛋白水解酶大量分泌，引起胰腺腺泡自溶。可通过严格控制注射剂量，注射频率控制成模时间以及模型的严重程度。 |
| 靶点 |
CCK receptor |
| 体外研究 |
Ceruletide is similar chemically and biologically to the human gastrointestinal hormones cholecystokinin-pancreozymin (CCK) and gastrin II. Ceruletide stimulates gallbladder contraction, pancreatic exocrine secretion, gastric secretion, and motility in the distal duodenum, jejunum, ileum and colon, while delaying gastric emptying and inhibiting motility in the proximal duodenum. Ceruletide in supramaximal but not in physiological doses activates NF-kappaB/Rel in vitro. This activation may induce a self-defending genetic program before the onset of cellular injury, which may prevent higher degrees of damage of pancreatic acinar cells after secretagogue hyperstimulation. |
| 体内研究 |
Ceruletide (0.4-0.5mcg/kg, i.v.; 3-4mcg/kg, s.c.) results in emesis and evacuation of the bowel in the intact conscious dog, and recovery is complete 15-30 min after i. v. administration and 2-4 hr after s.c. administration. Ceruletide (5-15ng/kg, i.v.) shows a marked spasmogenic effect on the pylorus of rats. Ceruletide also reduces blood pressure in anesthetized dogs. Ceruletide serum bile acid (SBA) stimulation circumvents exogenous and endogenous influences associated with postprandial (PP) SBA stimulation. Ceruletide SBA stimulation may perform as well as PP SBA stimulation in dogs with portosystemic shunt (PSS) and be more sensitive for the detection of hepatic dysfunction in dogs with upper respiratory disease (URD) |
推荐实验方法(仅供参考)
| Animal Administration |
Dogs
All dogs undergo serum bile acid (SBA) stimulation with food (5 kg BW 2 tablespoons) or 0.3μg/kg BW Ceruletide IM, respectively, on consecutive days. A diet of moderate protein content and with an increased concentration of fiber is chosen to minimize metabolic complications such as hepatic encephalopathy. Before each test, the dogs are fasted for 12 hours. Blood samples are drawn at baseline, 60 and 120 minutes after feeding, and 20, 30, and 40 minutes postinjection, respectively. The blood samples are collected in plain tubes and left to clot; they are then centrifuged at 6,500 ×g for 1 minute, and the serum is used to measure SBA by a colorimetric test with endpoint determination |
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